Effect of the method for the elimination of inhibitors present in Miscanthus giganteus hydrolysates on ethanol production effectiveness

Abstract The pretreatment of lignocellulosic material performed to improve substrate’s susceptibility enzymatic hydrolysis is usually accompanied by reactions leading the synthesis compounds that inhibit metabolic activity microorganisms. Their toxicity main obstacle successful bioconversion hydrolysates. identification these inhibitors and choice optimal detoxication method are crucial for improving efficiency fermentation processes. Material rinsing with water after processing a common practice. However, it generates losses, thus affecting contents saccharides in medium, which may turn trigger higher costs lignocellulose conversion ethanol other products added value. A study was undertaken determine effect selected methods an hydrolysate from Miscanthus giganteus on saccharide derivatives. experiment conducted Mucor rouxii DSM 1191 demonstrated usability based activated carbon. After 96-h hydrolysates, alcohol content post-reaction medium 14% than control wherein rinsed pretreatment. carried out Saccharomyces cerevisiae 7, NRRL 978 showed no positive impact alternative replacing synthesis. highest concentration this metabolite (2.04% ( v /v)) obtained experimental variant mentioned operation coupled hydrolysates using calcium hydroxide.